Isolation and Characterization of Nine Polymorphic Microsatellite Loci inPiper solmsianum(Piperaceae)
نویسندگان
چکیده
منابع مشابه
Isolation and characterization of nine polymorphic microsatellite loci from the desert locust, Schistocerca gregaria
Because of the scarcity of polymorphic genetic markers, only a few genetic studies on the population structure of the desert locust, Schistocerca gregaria, have been carried out. We isolated and characterized nine polymorphic dinucleotide microsatellite loci. These markers were evaluated using individuals from Niger and Senegal. Seven of these microsatellite markers are also applicable to the n...
متن کاملIsolation and characterization of nine polymorphic microsatellite loci in Piper solmsianum (piperaceae)1
UNLABELLED PREMISE OF THE STUDY Nine microsatellite (simple sequence repeat [SSR]) loci were characterized for natural populations of Piper solmsianum, a potential source of bioactive secondary metabolites, and analyzed to assess the levels of genetic diversity in this species. • METHODS AND RESULTS Based on an enriched library using the oligonucleotides (CT)8 and (GT)8, a total of 19 pair...
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Nine polymorphic microsatellite loci were developed and characterized for the temperate reef fish species, Hexagrammos decagrammus (kelp greenling). The number of alleles varied from three to 22 in a sample of 22 individuals from one population. Expected heterozygosities ranged from 0.354 to 0.979. These microsatellites allow us to investigate reproductive success of individuals, alternative ma...
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Other comments: Chromosomal location is unknown for the nine markers. The loci containing imperfect repeats (namely Sat4, Sat5 and Sat8) showed the greatest variability in allele size. Mutations leading to such differences in number of repeats probably involve processes other than mispairing slippage, which generates convergent mutations less frequently. Differences in allele size of Nine polym...
متن کاملIsolation and Characterization of Polymorphic Microsatellite Loci from Metapenaeopsis barbata Using PCR-Based Isolation of Microsatellite Arrays (PIMA)
The red-spot prawn, Metapenaeopsis barbata, is a commercially important, widely distributed demersal species in the Indo-West Pacific Ocean. Overfishing has made its populations decline in the past decade. To study conservation genetics, eight polymorphic microsatellite loci were isolated. Genetic characteristics of the SSR (simple sequence repeat) fingerprints were estimated in 61 individuals ...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2014
ISSN: 2168-0450
DOI: 10.3732/apps.1300092